Your email subscription to News & Announcements has been received. It is typically used in cell culture at concentration between 5mM to 30 mM. We have become experts in scientific operations, improving performance with sophisticated solutions and providing guidance on best practices. How is savings calculated?We 24: 53-68. Subscribe to our mail list and be the first to receive exclusive content from Hopax in your mailbox: Be the first to know about Hopax's special offers, new blog posts and relevant news about our industry. Structure, properties, spectra, suppliers and links for: HEPES, 7365-45-9. In addition to vwr.com, VWR, part of Avantor offers a state-of-the-art technology solutions portfolio. Design by 37design, Taiwan Hopax Chemicals Mfg. VWR provides the cell culture community with access to the most reliable supply of exceptional quality Fetal Bovine Serum: VWR Life Science Seradigm. Microbes Infect. PROTEIN A, G-PLUS & L AGAROSE IP REAGENTS, NUCLEOTIDES, PEPTIDES, PROTEINS & AMINO ACIDS, AUTORADIOGRAPHY FILM, TAPE AND PLASTIC WRAP, Buffer Components and Standard Laboratory Chemicals. 173, 111-115. Crystallization Plates, Hardware & Accessories, Crystallization grade HEPES buffer for formulating screens or for optimization, Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL). 2006. What is the critical micelle concentration (CMC)? Its quality is excellent and the price reasonable. Avantor Services provides a wide range of specialized services and digital solutions to help you solve complex challenges. A HEPES that is ethanesulfonic acid in which one of the methyl hydrogens is replaced by a 4-(2-hydroxyethyl)piperazin-1-yl group. 10: e0129876. The addition of 1025 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incub 25: 909-913. Exceptional skid resistance, both wet and dry, for dirty jobs and controlled environments. Quanta Biosciences and VWR are proud to fund a grant award for publication of research involving qPCR methodologies utilizing any Quanta Bioscience qPCR reagent in JoVE. To place an order using RMB or to ship to mainland China, please visit www.scbio.cn, Click on image or enlarge button to enlarge, • Recommended working concentration: 10-25 mM. Acta Crystallogr D 51, 393- 394, 1995. Chemother. Stoscheck, C.M. HEPES has been described as one of the best all-purpose buffers available for biological research. 238.300. g For a 20.0 mM solution from 238.3 g of solid, add 49999.8 ml of Water. 17: 651-653. The preparation of HEPES buffer solution, depending on the application, one is pure HEPES + NaOH and the other is HEPES + salt: First, HEPES + NaOH (500 mL) 119.15g HEPES is dissolved in 400mL distilled water, add 0.5~1M NaOH aqueous solution to adjust the pH, and then make it 500mL with distilled water. We are here to help you improve efficiencies and outcomes in your laboratory. You have the right to unsubscribe at any time by clicking the 'unsubscribe' link in any News & Announcement emails you receive. As our customers’ needs have evolved, so have our capabilities. Products > 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid, C1CN(CCN1CCO)CCS(=O)(=O)O CopyCopied, InChI=1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14) Hideshima, et. We are sad to see you go. I've been buying this product from Santacruz for the last couple of years. PMID: # 16531090  Guimarães, ET. Streamline your workflow storage with VWR Ultra- Low Temperature Upright Freezer. Available at https://link.springer.com/article/10.1007/BF00749399, 9 Soares, E. V., Duarte, A. P. R. S. and Soares, H. M. V. M. (2000) Chem. 107: PMID: # 26075403  Vicario, R. et al. Whether you are improving specific workstations, renovating your facility, or building a lab from scratch, the VWR Furniture Team is ready to help... REDISHIP Class l and Class ll biological safety cabinets are suitable for use with agents that require Biosafety Level 1, 2, or 3 containment. 238.300. g For a 20.0 mM solution from 238.3 g of solid, add 49999.8 ml of Water. Molecular Formula: C8H18N2O4S (HEPES solid, free acid) Molecular Weight: 238.3 (HEPES solid, free acid) CAS Number: 7365-45-9 Synonym: 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid solution This product is a 1.0 ± 0.1 M solution. © 2020 VWR International, LLC. Cell transplantation. © 2020 FORTUNE Media IP Limited All rights reserved. Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. Available from 2.5 to 18L, two styles & three temperatures with a broad range of accessories. Biochem. HEPES is normally used as a buffer in the pH range 6.8 to 8.2, not at pH 2. We set science in motion to create a better world. Optimize - Buffers HEPES is also known as 4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid. We’ve built our reputation on consistent, comprehensive mastery of day-to-day operations, allowing lab, clinical, and production environments to focus their high-value resources on core scientific priorities. Popular Answers (1) 23rd Jan, 2014. Med., 43, 1322–1327. https://www.hopaxfc.com/en/blog/the-9-best-biological-buffers-for-cell-culture (2011) Interactions of Biological Buffers: The Ubiquitous “Smart” Polymer PNIPAM and the Biological Buffers, MES, MOPS and MOPSO. PMID: # 19454526  Guimarães, ET. 5: 467-477. Not Intended for Diagnostic or Therapeutic Use. VWR is ready to support your production facility with reliable access to raw materials and essential supplies. Used under license. High-quality chemicals and services, customized to your product or manufacturing needs... VWR enables the advancement of science by providing high-quality chemicals and services, customized to your product or manufacturing needs. Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. Titrate HR2-585 to the desired pH between 6.8 - 8.2 using NaOH HR2-585 Measured pH Range: 5.4 - 5.5 at 25°C HR2-585 Measured Conductivity Range: 160.1 - 236.9 μS/cm at 25°C HR2-585 Measured Refractive Index Range: 1.37093 - 1.37131 at 20°C. Incompatible with strong oxidizing agents. Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA). In general, the optimal culture environment for most cells is pH 7.2-pH 7.4, and the buffering range of HEPES falls exactly in pH 6.8-pH 8.2, which is favorable for cell culture. The products you use, the products you need, the suppliers you trust for chromatography. Available at https://www.sciencedirect.com/science/article/pii/S0378517312000646?via%3Dihub, 11 Taha, M., Gupta, B. S., Khoiroh, I., Lee, M-J. Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. For Research Use Only in China. Available at https://pubs.acs.org/doi/abs/10.1021/ma201790c, 12 Zhao, G., and Chasteen, N. D. (2006) Anal. HEPES, CAS: 7365-45-9, is an N-substituted aminosulfonic acid buffer with an optimal buffering pH range of 6.0 to 8.5.. Cited in 31 publications Available at https://repositorium.sdum.uminho.pt/bitstream/1822/38712/1/document_19948_1.pdf, 8 Nagira, K., Hayashida, M., Shiga, M., Sasamoto, K., Kina, K., Osada, K., Sugahara, T. and Murakami, H. (1995) Cytotechnology, 17, 117–125. Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for... We found alternative products that can save you up to  per item-unit. Useful pH range of HEPES buffer 6.8 - 8.2. We use operational excellence to deliver solutions that enable research, testing, production, and commercialization across the globe. Freeze cells in tubes from 1 to 5mL using the Thermo Scientific™ Mr. Frosty™ Freezing Container at nearly -1°C/minute. pH: 9.0 ± 0.15 Storage: 2-8°C We collected academic references in which biological buffers were used for cell culture in order to help you understanding which buffers are more suitable for your applications. 2019. 56: 1247-55. These gloves offer the perfect combination of improved elongation with outstanding protection from chemotherapy drugs, and an FDA-approved low dermatitis potential claim. Available at https://www.ncbi.nlm.nih.gov/pubmed/21767488, 14 Bevans, C. G. and Harris, A. L. (1999) J. Biol. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. et al. 1990. PloS one. 1971. Sci Rep. 6: 32244. A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2. Sci Rep. 9: 4766. 2016. Meth. Stable. Create Account. (2012) A Two-Stage Reverse Dialysis In Vitro Dissolution Testing Method for Passive Targeted Liposomes, Int. al. For information visit, www.avantorsciences.com and find us on LinkedIn, Twitter and Facebook.